Resources

CRISPR screening protocols

(related to https://elifesciences.org/articles/81856 )

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Protocol 1: design and cloning of pooled libraries of programmed dual-sgRNAs

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Protocol 2: preparation of sgRNA sequencing libraries after CRISPR screens

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Protocol 3: cloning of arrayed dual-sgRNAs + corresponding Excel template

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Protocol 4: generation and validation of CRISPRi cell lines