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CRISPR screening protocols
(related to https://elifesciences.org/articles/81856 )
Protocol 1: design and cloning of pooled libraries of programmed dual-sgRNAs
Protocol 2: preparation of sgRNA sequencing libraries after CRISPR screens
Protocol 3: cloning of arrayed dual-sgRNAs
Protocol 4: generation and validation of CRISPRi cell lines