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Jost Lab publications (lab authors underlined)
* co-first author; ^ corresponding author

Replogle JM*, Bonnar JL*, Pogson AN*, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS^, Jost M^. Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. eLife 2022. Link and bioRxiv Link

Marco's publications as a post-doc

* co-first author; ^ corresponding author

Clark IC, Fontanez KM, Meltzer RH, Xue Y, Hayford C, May-Zhang A, D'Amato C, Osman A, Zhang JQ, Hettige P, Ishibashi JSA, Delley CL, Weisgerber DW, Replogle JM, Jost M, Phong KT, Kennedy VE, Peretz CAC, Kim EA, Song S, Karlon W, Weissman JS, Smith CC, Gartner ZJ, Abate AR^. Microfluidics-free single-cell genomics with templated emulsification. Nat Biotechnol. 2023 Mar 6. Link

Tolani B^, Celli A, Yao Y, Tan YZ, Fetter R, Liem CR, de Smith AJ, Vasanthakumar T, Bisignano P, Cotton AD, Seiple IB, Rubinstein JL, Jost M^, Weissman JS. Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice. Nature Biotechnology 2022. Link and bioRxiv Link

Zhang Y, Jost M, Pak RA, Lu D, Li J, Lomenick B, Garbis SD, Li CM, Weissman JS, Lipford JR, Deshaies RJ. Adaptive exchange sustains cullin-RING ubiquitin ligase networks and proper licensing of DNA replication. PNAS 2022. Link

Replogle JM*, Saunders RA*, Pogson AN, Hussmann JA, Lenail A, Guna A, Mascibroda L, Wagner EJ, Adelman K, Lithwick-Yanai G, Iremadze N, Oberstrass F, Lipson D, Bonnar JL, Jost M, Norman TM, Weissman JS. Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq. Cell 2022. Link and bioRxiv Link

Jost M*^, Jacobson AN*, Hussmann JA, Fischbach MA^, Weissman JS^. CRISPR-based functional genomics in human dendritic cells. eLife 2021 . Link and bioRxiv Link

Le Vasseur M, Friedman J, Jost M, Xu J, Yamada J, Kampmann M, Horlbeck MA, Salemi MR, Phinney BS, Weissman JS, Nunnari J. Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells. eLife 2021. Link and bioRxiv Link

Yan X, Stuurman N, Ribeiro SA, Tanenbaum ME, Horlbeck MA, Liem CR, Jost M, Weissman JS, Vale RD. High-content Imaging-based Pooled CRISPR Screens in Mammalian Cells. J Cell Bio 2021. Link and bioRxiv Link

Hawkins JS*, Silvis MR*, Koo BM, Peters JM, Osadnik H, Jost M, Hearne CC, Weissman JS, Todor H, Gross CA. Mismatch-CRISPRi Reveals the Co-varying Expression-Fitness Relationships of Essential Genes in Escherichia coli and Bacillus subtilis. Cell Systems 2020. Link and bioRxiv link

Hickey KL, Dickson K, Cogan JZ, Replogle JM, Schoof M, D’Orazio KN, Sinha NK, Hussmann JA, Jost M, Frost A, Green R, Weissman JS, Kostova KK. GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control. Mol Cell 2020. Link and bioRxiv link

Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS. Pharmaceutical-grade rigosertib is a microtubule-destabilizing agent. Mol Cell 2020. Link and bioRxiv link


Jost M*, Santos DA*, Saunders RA, Horlbeck MA, Hawkins JS, Scaria SM, Norman TM, Hussmann JA, Liem CR, Gross CA, Weissman JS. Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs. Nature Biotech 2020. Link and bioRxiv link

Norman TM*, Horlbeck MA*, Replogle JM, Ge AY, Xu A, Jost M, Gilbert LA, Weissman JS. Exploring genetic interaction manifolds constructed from rich single-cell phenotypes. Science 2019. Link and bioRxiv link

Chan MM*, Smith ZD*, Grosswendt S, Kretzmer H, Norman TM, Adamson B, Jost M, Quinn JJ, Yang D, Jones MG, Khodaverdian A, Yosef N, Meissner A, Weissman JS. Molecular recording of mammalian embryogenesis. Nature 2019. Link and bioRxiv link

Adamson B, Norman TM, Jost M, Weissman JS. Approaches to maximize sgRNA-barcode coupling in Perturb-seq screens. bioRxiv 2018. Link  (not submitted for publication)

Jost M^, Weissman JS^. CRISPR approaches to small molecule target identification. ACS Chem Bio 2018. Link

Jost M, Chen Y*, Gilbert LA*, Horlbeck MA*, Krenning L*, Menchon G*, Rai A*, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS. Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent. Mol Cell 2017. Link

Adamson B*, Norman TM*, Jost M, Cho MY, Nuñez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, Weissman JS. A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response. Cell 2016. Link

Marco's publications as a graduate and undergraduate student

Bian K, Lenz SAP, Tang Q, Chen F, Qi R, Jost M, Drennan CL, Essigmann JM, Wetmore SD, Li D. DNA repair enzymes ALKBH2, ALKBH3, and AlkB oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine and 5-carboxylcytosine in vitro. Nucl Acids Res 2019. Link

Padmanabhan S, Jost M, Drennan CL, Elías-Arnanz M. A New Facet of Vitamin B12: Gene Regulation by Cobalamin-Based Photoreceptors. Annu Rev Biochem 2017. Link

Wittenborn EC, Jost M, Wei Y, Stubbe J, Drennan CL. Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator. J Biol Chem 2016. Link

Chen F, Tang Q, Bian K, Humulock ZT, Yang X, Jost M, Drennan CL, Essigmann JM, Li D. Adaptive Response Enzyme AlkB Preferentially Repairs 1-Methylguanine and 3-Methylthymine Adducts in Double-Stranded DNA. Chem Res Toxicol 2016. Link

Jost M, Born DA, Cracan V, Banerjee R, Drennan CL. Structural Basis for Substrate Specificity in Adenosylcobalamin-Dependent Isobutyryl-CoA Mutase and Related Acyl-CoA Mutases. J Biol Chem 2015. Link

Jost M, Fernández-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elías-Arnanz M, Drennan CL. Structural Basis for Gene Regulation by a B12-Dependent Photoreceptor. Nature 2015. Link

Jost M, Simpson JH, Drennan CL. The transcription factor CarH safeguards use of adenosylcobalamin as a light sensor by altering the photolysis products. Biochemistry 2015. Link

Chang S, Fedeles BI, Wu J, Delaney JC, Li D, Zhao L, Christov PP, Yau E, Singh V, Jost M, Drennan CL, Marnett L, Rizzo CJ, Levine SS, Guengerich FP, Essigmann JM. N2,3-Ethenoguanine is a functional biomarker that induces the genetic changes typically seen in vinyl chloride associated liver tumors. Nucl Acids Res 2015. Link

Jost M, Cracan V, Hubbard PA, Banerjee R, Drennan CL. Visualization of a Radical B12 Enzyme with its G-protein Chaperone. PNAS 2015. Link

Sydor AM, Jost M, Ryan KS, Turo KE, Douglas CD, Drennan CL, Zamble DB. Metal-Binding Properties of Escherichia coli YjiA, a Member of the Metal Homeostasis-Associated COG0523 Family of GTPases. Biochemistry 2013. Link


Hamill MJ, Jost M, Wong C, Bene NC, Drennan CL, Elliott SJ. Electrochemical Characterization of Escherichia coli Adaptive Response Protein AidB. Intl J Mol Sci 2012. Link

Hamill MJ*, Jost M*, Wong C, Elliott SJ, Drennan CL. Flavin-Induced Oligomerization in Escherichia coli Adaptive Response Protein AidB. Biochemistry 2011. Link

Jost M, Zocher G, Tarcz S, Matuschek M, Xie X, Li SM, Stehle T. Structure-Function Analysis of an Enzymatic Prenyl Transfer Reaction Identifies a Reaction Chamber with Modifiable Specificity. JACS 2010. Link

Cupelli K, Müller S, Persson BD, Jost M, Arnberg N, Stehle T. Structure of Adenovirus Type 21 Knob in Complex with CD46 Reveals Key Differences in Receptor Contacts among Species B Adenoviruses. Journal of Virology 2010. Link

A complete list of peer-reviewed publications can be found at

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